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KMID : 0379120160440020103
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Korean Journal of Mycology
2016 Volume.44 No. 2 p.103 ~ p.107
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Species-specific Marker of Phytophthora pinifolia for Plant Quarantine in Korea
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Kim Na-Rae
Choi You-Ri
Seo Moon-Won
Song Jeong-Young
Kim Hong-GI
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Abstract
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To establish a rapid and accurate detection of Phytophthora pinifolia, which is a quarantine pathogenic fungus in Korea, a species-specific primer was developed based on the ras-related protein (Ypt1) gene. Species-specific primer based on the DNA sequences of Ypt1 gene amplified 193 bp polymerase chain reaction (PCR) product for P. pinifolia. The primer pair yielded the predicted PCR product size exactly in testing with target pathogen DNAs, but not from the other 10 species of Phytophthora and 14 species of other phytopathogenic fungi. The primer pair also showed only the species-specific amplification curve on realtime PCR on target pathogen DNA. The detection sensitivity of real time PCR using species-specific primer pair was 10 to 100 times higher than conventional PCR, with 1 to 10 pg/μL.
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KEYWORD
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Detection, Phytophthora pinifolia, Quarantine measure, Species-specific primers
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